Dataset title: Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION (version 2) DOI: 10.25917/ckw7-2m51 Principal Investigator Contact Information Name: Katherine Zulak Email: katherine.zulak@curtin.edu.au Institution: Curtin University ORCID: 0000-0003-0415-8975 Date of dataset publication: October 2025 Keywords: sequences, genes, fungal Primary dataset contact: Zulak, Katherine Grace; katherine.zulak@curtin.edu.au If necessary, further information or contact details may be found by emailing researchdata@curtin.edu.au +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ DATA INFORMATION Data description: Samples are barley (Hordeum vulgare) leaves infected with Pyrenophora teres collected from paddocks in Western Australia. The azole fungicide target gene Cyp51 was amplified from the samples using unique molecular identifiers according to Zulak et al., (2024) Scientific Reports 14:1 6285. Amplicons were subsequently sequenced with the Oxford Nanopore Technologies MinION sequencer using the Native Barcoding Kit V14 and the MIN114 flow cell. The resulting data file is a .fastq file containing sequence information. Collected under CCDM Project A3.1 Files types: .fastq.gz; .pod5 (raw data) Geographic information (if relevant): Western Australia +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ REUSE INFORMATION Recommended citation: Zulak, K. (2025). Amplicon sequencing of Cyp51 alleles from Pyrenophora teres using the Oxford Nanopore Technologies MinION (version 2) [Data set]. Curtin University. https://doi.org/10.25917/CKW7-2M51 This dataset is provided under a CC BY 4.0 license. More details can be found at https://creativecommons.org/licenses/by/4.0/